Endocrine Abstracts

Purpose: For vaginoplasty of Rokitansky syndrome, we use oxidized regenerated cellulose instead of bio-craft that has been used in the past because it requires a less invasive and more physiological surgery. However, the mechanism of epithelialization of this procedure is still not clear. There is a report that differentiation and proliferation of the vaginal epithelium of newborn mice is promoted by administration of basic fibroblast growth factor (bFGF).<p class="abstext...

Context: 18-Oxocortisol (18-oxoF) is a derivative of cortisol that is produced by aldosterone synthase (CYP11B2). The potential for this steroid, as a biomarker for differentiating patients with aldosterone-producing adenoma (APA) from those with idiopathic hyperaldosteronism (IHA), has not been examined.Objectives: We measured 18-oxoF, aldosterone (A) and cortisol (F) in plasma from adrenal vein sampling (AVS) patients. We compared 18-oxoF and 18-oxoF/F...

18-Oxocortisol (18-oxoF) is a derivative of cortisol (F) that is produced by aldosterone synthase (CYP11B2), and its clinical usefulness has not been clarified. We prospectively measured 18-oxoF, using highly sensitive liquid chromatography-tandem mass spectrometry (LC/MS/MS), in peripheral plasma of patients with primary aldosteronism (PA) who underwent adrenal venous sampling (AVS) to differentiate aldosterone producing adenoma (APA) from bilateral hyperplasia. The study was...

Background: The human adrenal cortex is composed of 3 distinct zones: the zona glomerulosa (ZG), the zona fasciculata (ZF), and the zona reticularis (ZR). Steroidogenic enzymes are expressed in a zone-specific manner. The promoters of CYP11B1 and CYP11B2 possess a number of target sites for DNA methylation that is closely associated with gene silencing.Methods and Materials: Adrenals from 5 autopsy subjects were assessed for immunohistrochemically define...